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PBiC Workshop Registration

The Bioinformatics Core hosts workshops of relevance to scientists in the Penn community on topics related to bioinformatics. If you would like to suggest a topic, please send email to workshops@pcbi.upenn.edu and we will consider adding your topic to the schedule.

Upcoming workshops:



Past workshops

Most workshops are offered twice a year, and sessions are usually scheduled 4 - 8 weeks in advance.


Microarray Workshop I - Using Microarrays to Find Differentially Expressed Genes - John Tobias
  • Experimental Design
    • Replicates
    • One Color vs Two Color
    • Statistical Power
    • Laboratory Process Control
    • Batch Effect
  • Pixels to Genes
    • Algorithm choices and example
  • Files on your data CD
  • Analytical Sample QC
    • Sample Clustering
    • Sample Principle Components Analysis
  • Differential Expression Analysis
    • Algorithm Comparison
    • False Discovery Rate Control

Sequence Analysis Strategies I (sequence alignment) - Brian Brunk

A practical workshop that will address the basic questions one can ask starting with a sequence, the types of algorithms that are available, resources for running those algorithms and interpreting the results.

  • Questions that can be addressed
    • What can I learn about my sequence?
    • What other sequences exist with similarity to my sequence?
  • What algorithms are available?
    • BLAST, BLAT, motif identification, genomic comparison, etc.
  • Resources available on the web and your desktop.
  • Multiple sequence alignment including psi-blast.
  • Introduction to phylogenetic analyses.

Microarray Workshop II - Beyond the Gene List - Annotation, Clustering, Sample Class Discovery, Pathway Analysis - John Tobias
  • Mining for similarity to expression pattern of interest
  • Clustering
    • Discovery of sample sub-classes
    • Gene Clustering Algorithms
    • Comparing 2 clustering methods
  • Annotation Mining
    • Statistics of list overlap (over-representation)
    • EASE tool
  • Pathway Analysis
    • Ingenuity Pathway Analysis
    • Public Resources
  • Public Expression Data Repositories
    • Searching for datasets
    • Submission of experimental data (MIAME)

Functional Genomics Resources in Model Organisms - Multiple Speakers
  • 1:00 - Introduction (Brian Brunk and Maja Bucan)
  • 1:15 - Yeast - Chris Burd, Department of Cell and Developmental Biology
  • 1:45 - Arabidopsis - Scott Poethig, Department of Biology
  • 2:15 - Worm - Meera Sundaram, Department of Genetics
  • 2:45 - Fly - Brian Calvi, Department of Genetics
  • 3:15 - Zebrafish - Michael Granato, Department of Cell and Developmental Biology
  • 3:45 - Mouse - Maja Bucan, Department of Genetics

Each speaker will give a 20 minute presentation on functional genomics resources in their favorite organism with 10 minutes between sessions. Please feel free to attend individual presentations. The presentations will have a similar format and will address the following types of questions:

  • Are there orthologs of this gene?
  • What is the location of this gene in the genome or on the genetic map?
  • Are mutants in this gene available?
  • Is there a reported RNAi phenotype for this gene?
  • Are there any known binding partners?

Vector NTI Lecture and hands-on workshop - Rob Mervis (InVitrogen)

Rob Mervis from InVitrogen will be here Friday, December 3 to present training sessions for VectorNTI advance, a powerful suite of sequence analysis tools. VectorNTI is available to the UPenn community via subscription to the sequence tools that PBiC provides. There will be a lecture (with question and answer times) in the morning from 9:00 - 11:30 in the BRBII/III Auditorium (registration not required) and a hands on workshop at 1:00 PM in 1311 Blockley (you must register below). We have 10 computers available (first come first served). The training room is wireless enabled so please bring your own laptop if possible. We will help you install VectorNTI so you can actively participate in the training session.


Vector Xpression and Pathblazer Training - Kevin Groch (InVitrogen)
  • Overview
    • General architecture
    • Workflow through the product
  • Data Analysis and Mining
    • Basic (DeRisi et al., 1997 Science 278: 680)
      • Interface elements
      • Identifying differential gene expression
      • Interacting with external programs and data sources
    • Advanced (Hedenfalk et al., 2001, N Engl J Med 344; 539)
      • Classification (clustering, PCA, etc)
      • Export of result
  • Statistics
  • Data Normalization
    • Two channel
    • Affymetrix
  • Data Import
    • Fluorescence intensities (two channel and Affymetrix)
    • Annotations and hyperlinking
    • Pathways
  • Automation
    • File formats
    • Analyses
  • Extensibility
    • Custom analyses in R and MatLab

LEARN ABOUT DS GENE AND MACVECTOR - Accelrys

Accelrys' easy-to-use sequence analysis solutions for Windows and Macintosh. Come to one of these sessions and experience the new wave of bioinformatics tools from Accelrys. DS Gene and MacVector provide everything you need for day-to-day sequence analysis and documentation in a single compact easy to use application;

  • Very shallow learning curve — get new graduate students up to speed in just a few minutes
  • Full sequence editing capabilities with support for all major file formats
  • Highly customizable graphic feature maps
  • Integrated on-line BLAST and Entrez searching
  • Simple point and click clone construction and documentation
  • Numerous nucleic acid property plots and analyses — ORFs, Restriction Enzymes,composition, binding sites
  • Primer design
  • Multiple sequence alignment and editing
  • Phylogenetic analysis
  • Protein sequence analysis
  • and lots more...

Workshops will include demonstrations of the software, answering your questions and hands on use of these packages (bring your own wireless enabled laptop....a limited number of PCs will be available for the DS Gene session). You can even bring some of your own data to ask specific questions and begin your analyses.

  • Session I (10:00 - 11:30) will cover MacVector only (Macintosh)
  • Session II (1:00 - 2:30) will cover DS Gene only (Windows)
  • Session III (3:00 - 4:30) will be a MacVector overflow session. Register for this session only after Session I is full.

Introduction to UNIX and PERL - PBiC Staff

This workshop is for users new to the UNIX environment and/or Perl. We will tell you about using the UNIX command line (MacOSX is UNIX), why this is worth learning and what sorts of tools are available. The second half of this workshop will be an introduction to the perl programming language. Topics that will be covered include:

  • Accessing a UNIX machine
    • Using MacOSX terminal
    • Connecting to a UNIX server (from Windows and Macintosh)
    • Your environment
  • Unix commands
    • Documentation
    • File / directory management
    • File display and editing
    • Running programs and managing processes
  • Why consider learning Perl as a biologist?
  • Example tasks that can be addressed using Perl.
  • Creating a simple but useful Perl script.
    • We will cover basic programming concepts as we work through this example.
  • Resources for learning Perl.

Using The InQuiry Sequence Analysis Web Tools - PBiC Staff

This workshop focuses on sequence analysis using the BioTeam InQuiry tools which are available via a web browser at no charge to persons with a PennKey at https://inquiry.bioinformatics.upenn.edu/. This package has many sequence analysis programs that make a nice alternative to desktop analysis packages and in many cases exceed what one finds in a desktop package. We will present the different tools that are available and demonstrate how to use this powerful package. Tools available include:

  • multiple sequence alignment
  • gene finding
  • phylogenetic reconstruction
  • database searching (BLAST databases updated weekly)
  • EMBOSS package (many sequence analysis tools...click here for documentation.

Introduction to UNIX for beginners - PBiC Staff

This workshop is for users new to the UNIX environment and should be taken before the Perl workshops if possible for users without UNIX experience. Topics that will be covered include:

  • Accessing a UNIX machine
    • Using MacOSX terminal
    • Connecting to a UNIX server (from Windows and Macintosh)
    • Your environment
  • Unix commands
    • Documentation
    • File / directory management
    • File display and editing
    • Running programs and managing processes

Perl Programming Part I - PBiC Staff

This first Perl workshop (lecture format) is intended to introduce persons to basic programming concepts that will allow them to write simple Perl scripts. Example scripts will be provided.

  • Managing inputs and outputs
  • Variables
  • Loops
  • Conditionals

Perl Programming Part II - PBiC Staff

A continuation of Perl Programming Part I. Should provide the basis for writing most perl scripts that manage data. We will work through some useful but short examples.

  • Regular Expressions
  • Perl functions
  • Subroutines (methods)
  • Programming guidelines (writing understandable code)

Perl Programming Part III (Using Objects) - PBiC Staff

This workshop will be focused on object oriented programming and using Perl objects. Particular emphasis will be given to using the BioPerl objects which provide much functionality for Bioinformatics applications.

  • What are objects in programming?
  • Using Perl objects
  • Creating your own objects
  • Using BioPerl objects

Ingenuity Pathway Analysis - Deborah Riley, Ph.D.

Join your colleagues for a seminar and scientific discussion focused on helping you understand biological mechanisms underlying your experimental data. Learn how scientists can simultaneously analyze multiple genomic and proteomic datasets to rapidly gain biological insight and understanding.

Location

Austrian Auditorium
Clinical Research Building, 1st Floor
University of Pennsylvania
415 Curie Blvd.
Philadelphia, PA 19104

 

Speaker

Deborah Riley, PhD.
Senior Application Scientist, Ingenuity

 

Date

June 9, 2005
10:00 am to 12:00 noon

Scientists are using Ingenuity Pathways Analysis to extend the statistical analysis of their expression data allowing them to make better and faster decisions. The web-delivered application makes use of the Ingenuity Pathways Knowledge Base, the world’s largest curated database consisting of millions of individually modeled relationships between proteins, genes, complexes, cells, tissues, drugs, and diseases.

Deborah Riley, Ph.D. will present a case study in which this technology is being used to gain insight into the biological functions and molecular mechanisms that distinguish different stages of prostate cancer.

For questions or more information contact Margaret DiFilippo,

mdifilippo@ingenuity.com,

609-971-8677



Introduction to Homology Modeling of Protein Structure using DeepView and Swiss-Model - Yu Sun

This workshop is for biologists who would like to build 3D model of a target protein based on the sequence similarity between the target and a “template” 3D structure. We will get you familiar with DeepView (Swiss-PDBviewer) and Swiss-Model in order to carry out tasks including:

  • Active site analysis
  • Single residue mutation modeling
  • Structures comparison
  • Homology template(s) selection
  • Generate initial model
  • Model optimization
  • Assessing model quality

Familiarity with other 3D structure analysis tools such as Cn3D, rasmol, VMD would be helpful but not required. For people who attended NCBI’s workshop for Cn3D, you already learned how to search for proper structure templates using NCBI tools. This workshop would help you extend that knowledge into homology model building based on selected templates. In addition, you will learn how to use DeepView, which is more powerful than Cn3D in 3D structure analysis.


Analyzing promoters in sets of co-expressed genes - Brian Brunk

This workshop will focus on the analysis of gene promoters to identify transcription factor binding sites, particularly those that may be involved in the condition specific expression of a set of genes. We will explore a variety of bioinformatics tools both on the web and available at PBiC. Topics covered will include:

  • Extracting the promoter sequences.
  • Leveraging promoters from orthologous genes to identify conserved sites.
  • Identifying known sites that are over-represented in the target set.
  • Identifying motifs de novo based on sequence similarity within the promoters of the target set.

Spring Genomics Workshop - Don Baldwin

Spring Genomics Workshop

Wednesday, March 19, 2008
9:30am, BRB II/III Auditorium

Free Box Lunch, Registration Required
Latest Technologies - Access to Local Resources – Application Examples

9:30am: Proteomics – Chao-Xing Yuan, PhD

10:20am: Tissue Microarrays and Laser Capture – Daniel Martinez

11:10am: High-throughput sequencing – Don A. Baldwin, PhD

Noon: Vendor Displays, Core Facility Posters, Lunch

Online registration is closed. You may still check in at the BRB Auditorium on the morning of the workshop.


Fall Genomics Workshop - Don Baldwin

Fall Genomics Workshop

Friday October 26, 2007
9:30am, BRB II/III Auditorium

Free Box Lunch, Registration Required
Latest Technologies - Access to Local Resources – Application Examples

9:30am: Research & Clinical Applications of Genomics Technologies – Don Baldwin, Ph.D.

10:00am: DNA copy number assays, tiling arrays – Eric Rappaport Ph.D.

10:30am: Experimental Design, Bioinformatics services – John Tobias Ph.D.

11:00am: High-throughput screening – Scott Diamond Ph.D.

11:40am: Vendor Displays

Noon: Lunch, Core Facility posters, Vendor displays


Spring Genomics Workshop - Don Baldwin

PROTEOMICS  Chao-Xing Yuan, PhD  Proteomics Core Facility

Differential 2-D gel assays, chromatography and mass spectrometry methods, data analysis.

SNP GENOTYPING  Don Baldwin, PhD  Molecular Diagnosis and Genotyping Facility

Single nucleotide polymorphism genotyping using individual, multiplex and whole genome assays.

TISSUE MICROARRAYS  Alex Judkins, PhD  CHOP Pathology Core Laboratories

Creation and screening of multiplex tissue section microarrays.


Sequencher Workshop - Naomi Thomson

Sequencher

DNA Sequence Analysis Workshop

1311 Blockley Hall

University of Pennsylvania

Tuesday Morning February 19th

10:00 – 12:00

CHOP (909 Abramson) Session Added: Tuesday Afternoon February 19th

2:00 - 4:00

or

Wednesday Afternoon February 20th

2:00 - 4:00

For years Sequencher has been a favorite tool for assembly and contig editing. It still is, but Sequencher also does much more. This workshop will demonstrate both Sequencher’s foundation functions and its newer functions.

The Foundation

  • Import
  • Trim
  • Assemble
  • Edit
  • Report

The New

  • Automation for large numbers of samples
  • Import of GenBank annotation including SNPs and exons
  • Polymorphism detection for both nucleic and amino acid differences
  • Advanced Reporting, including Variance Reporting and Population Reports

Gene Codes invites you to customize this workshop for your needs. If you have a data set that you can share, we can make this part of the workshop, or we can work with you one on one. Please write to Naomi Thomson directly to contribute data to the workshop or for an appointment.

Register early, because space is limited, by clicking below on "Register for workhop." You will then be able to choose the session you wish to attend.


MacVector 10 - Kevin Kendall

Kevin Kendall, CEO of MacVector, Inc. is coming to UPenn to introduce release 10 of MacVector. The program is available as part of PBiC's Sequence Analysis Suite via subscription to the sequence tools. Please register for one of the 3 90 minute sessions listed below.

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